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Registros recuperados: 117
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Le Polymorphisme Electrophoretique : Un Moyen d'Etude des Populations. ArchiMer
Pichot, Paul; Pichot, Yves.
The study of biochemical polymorphisms has been known for some years as an important development. The use of a technique called electrophoresis is at the origin of the progress realized in this science. The electrophoretic detection of genetical heterogeneity existing at the level of some biochemical characters of animal species presents an important theorical and practical interest.
Tipo: Text Palavras-chave: Pisces; Overfishing; Stock assessment; Population genetics; Electrophoresis; Biochemical analysis; Analytical techniques.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/publication-1909.pdf
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Variabilite genetique de quelques especes de crevettes Peneides d'interet aquacole ArchiMer
Ko, G; Pasteur, N; Bonhomme, Francois; Liao, D.
Thirteen populations belonging to seven species of Penaeid prawns (Crustacea, Decapoda) were analyzed at 29 protein loci. The authors show that both inter and intrapopulational genetic diversity are very low. These results are discussed in view of their use as guidance for aquaculture programs.
Tipo: Text Palavras-chave: Penaeus kerathurus; Penaeus orientalis; Penaeus indicus; Penaeus monodon; Penaeus stylirostris; Penaeus japonicus; Penaeidae; Malacostraca; Prawn culture; Aquaculture development; Sexual reproduction; Biopolymorphism; Population genetics; Polymorphisme enzymatique; Variabilité génétique; Crevettes Pénaeides; Crustacés.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1186.pdf
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Constitution of aquacultural stocks: Genetic aspects. ArchiMer
Chevassus, B.
Before reaching of production level justifying an elaborated genetic improvement program, the new aquaculture species deserve a minimal strategy to avoid the consequences of uncontrolled genetic phenomena. Three main aspects will be discussed: initial constitution of the broodstock; maintenance of the broodstock; selection of the broodstock.
Tipo: Text Palavras-chave: Oncorhynchus keta; Salmo salar; Salmo trutta; Salmonidae; Pisces; Marine molluscs; Marine fish; Sex ratio; Females; Males; Eggs; Larvae; Spawning populations; Aquaculture; Population genetics.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1442.pdf
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Analyse genetique et physiologique des interactions hote-parasite: le systeme prosorhynchus squamatus - Mytilus ArchiMer
Coustau, Christine.
Tipo: Text Palavras-chave: Mytilidae; Prosorhynchus squatamus; Host oreferences; Population genetics; Physiology; Parasitic diseases; Mytilus.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/these-1571.pdf
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Genética bioquímica poblacional del delfín Tonina Tursiops truncatus (Montagu, 1821) de Cuba. OceanDocs
Pérez-Cao, H..
Existe un grado de diferenciación entre las 2 poblaciones analizadas, así mismo hay una tendencia no significativa hacia un exceso de heterocigóticos en la población total.
Tipo: Theses and Dissertations Palavras-chave: Population genetics; Biochemistry; Marine mammals.
Ano: 1996 URL: http://hdl.handle.net/1834/4595
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Karyological, biochemical, and physiological aspects of Callophysus macropterus (Siluriformes, Pimelodidae) from the Solimões and Negro Rivers (Central Amazon) BJMBR
Ramirez-Gil,H.; Feldberg,E.; Almeida-Val,V.M.F.; Val,A.L..
Karyological characteristics, i.e., diploid number, chromosome morphology and nucleolus organizer regions (NORs), biochemical characteristics, i.e., electrophoretic analysis of blood hemoglobin and the tissue enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and phosphoglucose isomerase (PGI), and physiological characteristics, i.e., relative concentration of hemoglobin and intraerythrocytic concentrations of organic phosphates were analyzed for the species Callophysus macropterus collected from Marchantaria Island (white water system - Solimões River) and Anavilhanas Archipelago (black water system - Negro River). Karyological and biochemical data did not reveal significant differences between specimens...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callophysus; Fish; Amazon; Population genetics; Adaptation.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100014
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International study on <i>Artemia</i> : LX. Allozyme data suggest that a new <i>Artemia</i> population in southern Chile (50° 29' S; 73° 45' W) is <i>A. persimilis</i> OMA
Gajardo, G.; Mercado, C.; Beardmore, J.A.; Sorgeloos, P..
An <i>Artemia</i> population from a site recently described in Chile and located in a semi-arid and humid hydrographical zone (Torres del Paine National Park), was electrophoretically characterized and cross-bred (followed through <i>F</i><sub>3</sub>) to reference samples of <i>A. franciscana</i> (San Francisco Bay, California, USA) and <i>A. persimilis</i> (Buenos Aires, Argentina), which are the species most likely to be found in Chile. The population exhibited high levels of genetic variability as judged by the percentage of polymorphic loci (<i>P</i>=53.3), mean number of alleles per locus (<i>n</i>=2.2) and mean heterozygosity (<i>H</i>=0.176). Based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allopatric populations; Allozymes; Genetic isolation; Hybridization; Population genetics; Chile.
Ano: 1999 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=4016
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Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe ArchiMer
Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre.
The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and...
Tipo: Text Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata.
Ano: 2000 URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf
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Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland Naturalis
Shinn, A.P.; Banks, B.A.; Tange, N.; Bron, J.E.; Sommerville, C.; Aoki, T.; Wootten, R..
Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon ( Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed...
Tipo: Article / Letter to the editor Palavras-chave: Sea louse; Copepod; Crustacea; Atlantic salmon; Sequencing; 18S ribosomal DNA; Internal transcribed spacer; Population genetics.
Ano: 2000 URL: http://www.repository.naturalis.nl/record/534343
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Population Genetic Aspects of Pollinator Decline Ecology and Society
Packer, Laurence; York University; bugsrus@yorku.ca; Owen, Robin; Mount Royal College and University of Calgary; rowen@mtroyal.ab.ca.
We reviewed the theory of conservation genetics, with special emphasis on the influence of haplodiploidy and other aspects of bee biology upon conservation genetic parameters. We then investigated the possibility that pollinator decline can be addressed in this way, using two meta-analytical approaches on genetic data from the Hymenoptera and the Lepidoptera. First, we compared levels of heterozygosity between the orders. As has been found previously, the haplodiploid Hymenoptera had markedly lower levels of genetic variation than the Lepidoptera. Bees had even lower levels, and bumble bees, in particular, often seemed almost monomorphic genetically. However, the statistically confounding effects of phylogeny render detailed interpretation of such data...
Tipo: Peer-Reviewed Reports Palavras-chave: Bumble bees; Effective population size; Gene flow; Haplodiploidy; Heterozygosity; Hymenoptera; Lepidoptera; Meta-analysis; Pollinator decline; Population fragmentation; Population genetics.
Ano: 2001
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Danko Brncic and the flower-flies RChHN
CARSON,HAMPTON L..
This is a personal essay commemorating Brncic as a keen naturalist/evolutionist in the best Darwinian sense. His innate curiosity, so important in a scientist, led him to the discovery of cenain unique drosophilid flies in Chile. The life cycle of one species of flower-fly (Drosophila flavopilosa) is of special interest. Females oviposit in fresh flowers of a solanaceous plant that is widespread in Chilean valleys. Twenty years of combined ecological and genetic work, carried out on this species, pioneers the use of a highly specialized Drosophila species in the study of population genetics and evolution. Many eco-genetic features are revealed that are important supplements to observations made on the widely-studied generalist species of the genus
Tipo: Journal article Palavras-chave: Flower-breeding Drosophila; Genetic specialization; Ecology; Population genetics; Evolution.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000008
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Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst...
Tipo: Text Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf
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Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers PAB
Barcelos,Edson; Amblard,Philippe; Berthaud,Julien; Seguin,Marc.
The objective of this work was to evaluate the genetic diversity, its organization and the genetic relationships within oil palm (Elaeis oleifera (Kunth) Cortés, from America, and E. guineensis (Jacq.), from Africa) germplasm using Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP). In complement to a previous RFLP study on 241 E. oleifera accessions, 38 E. guineensis accessions were analyzed using the same 37 cDNA probes. These accessions covered a large part of the geographical distribution areas of these species in America and Africa. In addition, AFLP analysis was performed on a sub-set of 40 accessions of E. oleifera and 22 of E. guineensis using three pairs of enzyme/primer combinations. Data were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elaeis oleifera; Elaeis guineensis; Population genetics; Palm species; Genetic resources; Molecular chacterization.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000800008
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Genetic structure of honeybee populations from southern Brazil and Uruguay Genet. Mol. Biol.
Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008
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Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci Genet. Mol. Biol.
Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de.
A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population genetics; Plant breeding; Molecular markers.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022
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Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae) from the Brazilian Atlantic Forest Genet. Mol. Biol.
Maroja,L.S.; Almeida,F.C.; Seuánez,H.N..
We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus) and high levels of both observed (0.718-0.789) and expected (0.748-0.832) heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q) and R ST (r) analogues was 0.037 for q and 0.060 for r. There was significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Migration; Population genetics; Schistosoma mansoni host; Sigmodontinae.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400002
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Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes) ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf
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Color polymorphism and allele frequency in a Brazilian population of the sunflower caterpillar Chlosyne lacinia saundersi (Doubleday) (Lepidoptera: Nymphalidae) Neotropical Entomology
Lopes-da-Silva,Marcelo; Casagrande,Mirna M..
The aim of this work was at calculating allele frequency color polymorphism in a population of sunflower caterpillar, Chlosyne lacinia saundersi (Doubleday) from Londrina, Paraná State, Brazil. Allele frequency in insect populations can be used as genetic marker to compare populations from different geographical and host origins. There are three phenotypes conditioned by two loci interacting epistatically. The phenotypes are: rufa (oranged colored larvae), bicolor (black larvae with dorsal orange stripes) and nigra, larvae with the body entirely black, sometimes with dorsal yellow dots, best seen in the fourth and fifth instars. Samples were taken independently in an attempt to obtain all combinations of crossing among genotypes. The genetic mechanism of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epistasis; Population genetics; Ecological genetics; Phenotype.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000100025
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Intrapopulation fixation index dynamics in finite populations with variable outcrossing rates Scientia Agricola
Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland.
The intrapopulation fixation index ( f ) is inversely related to the outcrossing rate (t). Results obtained from data on molecular markers of natural populations have shown that these values are highly variable, even when measured in the same group of individuals. It is thus suggested that factors besides those described in Wright's genetic equilibrium must be operating. Using simulated data sets this study shows that the finite size condition of a population is sufficient to spread the estimated f values along a range at equilibrium, as opposed to keeping them at the theoretical equilibrium point. The variation in outcrossing rates can amplify this range considerably. Correlation between estimated f values obtained from different loci in this condition...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population genetics; Inbreeding; Computer simulation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200015
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Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae) Genet. Mol. Biol.
Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013
Registros recuperados: 117
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